Amorphous Trisodium Sacubitril Valsartan and process for its preparation

ABSTRACT

The present invention provides for crystalline Form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenyl-methyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt, crystalline Form B2 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt, a novel process for the preparation of crystalline Form B of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt.

RELATED APPLICATION

This application claims the benefit of priority of our Indian patent applications IN 201641037145 filed on Oct. 28, 2016, IN 201641038689 filed on Nov. 11, 2016 and IN 201641043910 filed on Dec. 22, 2016 which are incorporated herein by reference.

TECHNICAL FIELD

The present invention relates to methods for preparation of amorphous form of Sacubitril Valsartan Trisodium complex represented by the structural Formula-I and processes for the preparation thereof.

The present invention also relates to novel crystalline forms of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt [Sacubitril sodium] represented by the structural Formula-II and processes for the preparation thereof.

The present invention also relates to novel crystalline forms of (S)—N-(5 1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt [Valsartan disodium] represented by the structural Formula-I and processes for the preparation thereof.

BACKGROUND AND PRIOR ART OF THE DISCLOSURE

ENTRESTO÷ is a combination of sacubitril, a neprilysin inhibitor, and valsartan, an angiotensin II receptor blocker, indicated to reduce the risk of cardiovascular death and 10 hospitalization for heart failure in patients with chronic heart failure (NY HA Class II-IV) and reduced ejection fraction.

Sacubitril sodium is disclosed in U.S. Pat. No. 5,217,996 A along with methods for preparation of Sacubitril sodium.

Journal of Medicinal Chemistry, 1995, 38 (10), pp 1689⁻1700 discloses the process for preparation of Sacubitril sodium.

CN105503638A discloses process of preparation of Sacubitril sodium using ethanol and tetrahydrofuran (1:1) mixed solvent.

CN105693543 discloses various salts of Sacubitril like Sacubitril potassium, magnesium Sacubitril, Sacubitril t-butylamine salt, Sacubitril ethanolamine salt, Sacubitril piperazine, Sacubitril calcium salt, Sacubitril tromethamine salt, Sacubitril triethanolamine salt. This patent application also discloses Sacubitril magnesium amorphous of X-ray powder diffraction pattern.

CN105837464 A discloses various crystalline forms of Sacubitril sodium such as Form A, Form B, Form C and Form D along with amorphous form.

This Chinese patent application discloses Sacubitril sodium of crystal line Form A which is characterized by X-ray powder diffraction pattern (using Cu—K radiation) the characteristic diffraction peaks corresponding 2::values are 6.0 ě 0.2 é, 6.6 ě 0.2 é, 9.0 ě 0.2 é, 12.2 ě 0.2 é, 13.2 ě 0.2 é, 15.1 ě 0.2 é, 17.1 ě 0.2 é, 17.8 ě 0.2 é, 18.3 ě 0.2 é, 19.2 ě, 0.2 é, 19.7 ě 0.2 é, 20.2 ě 0.2 é, 20.8 ě 0.2 é, 21.4 ě 0.2 é, 21.6 ě 0.2 é, 22.6 ě 0.2 é, 26.7 ě, 0.2 é.

This Chinese patent application discloses Sacubitril sodium of crystalline Form B which is characterized by X-ray powder diffraction pattern (using Cu—K radiation) the characteristic diffraction peaks corresponding 2::values are 6.1 ě 0.2 é, 7.1 ě, 0.2 é, 7.7 ě 0.2 é, 11.8 ě 0.2 é, 12.3 ě 0.2 é, 12.6 ě 0.2 é, 13.6 ě 0.2 é, 15.4 ě 0.2 é, 16.4 ě 0.2 é, 19.9 ě 0.2 é, 20.1 ě 0.2 é, 23.7 ě 0.2 é, 24.0 ě 0.2 é, 24.8 ě 0.2 é, 25.9 ě 0.2 é, 26.2 ě 0.2 é.

This Chinese patent application discloses Sacubitril sodium of crystalline Form C which is characterized by X-ray powder diffraction pattern (using Cu—K radiation) the characteristic diffraction peaks corresponding 2::values are 6.3 ě 0.2 é, 7.3 ě 0.2 é, 12.7 ě 0.2 é, 14.7 ě 0.2 é, 15.9 ě 0.2 é, 18.5 ě 0.2 é, 19.2 ě 0.2 é, 22.4 ě 0.2 é, 22.7 ě 0.2 é, 23.6 ě 0.2 {acute over ( )}, 27.0 ě 0.2 é.

This Chinese patent application discloses Sacubitril sodium of crystal line Form D which is characterized by X-ray powder diffraction pattern (using Cu—K radiation) the characteristic diffraction peaks corresponding 2::values are value 6.3 ě 0.2 é, 7.3 ě 0.2 é, 12.0 ě 0.2 é, 12.8 ě 0.2 é, 16.6 ě 0.2 é, 17.0 ě 0.2 é, 18.5 ě 0.2 é, 20.0 ě 0.2 é, 21.7 ě 0.2, 22.5 ě 0.2 ě, 23.9 ě, 0.2 é, 26.3 ě 0.2 é, 27.1 ě 0.2 é.

PCT Publication No. WO2016074651A1 discloses various crystalline Sacubitril amine salts. This PCT publication discloses the crystalline salt of Sacubitril with cyclohexyl amine, the characteristic diffraction peaks corresponding 2::values are 7.4, 15.0, 18.0, 19.9, 21.6 (éě 0.2é2:), the crystalline salt of Sacubitril with isopropyl amine, the characteristic diffraction peaks corresponding 2::values are 3.1, 9.5, 12.6, 18.5, 24.6 (é ě 0.2é 2:), the crystalline salt of Sacubitril with isopropyl amine, the characteristic diffraction peaks corresponding 2::values are 8.0, 9.8, 16.1, 17.8, 20.2 (éě 0.262:).

PCT Publication No. WO2016074651A1 discloses various crystalline and semicrystalline forms of Sacubitril calcium salt. This PCT discloses the crystalline form I of crystalline Sacubitril calcium salt with characteristic diffraction peaks corresponding 2::values are 3.6, 6.4, 8.4, 10.8, 14.5, 17.7, 20.6 and 22.5 (ě 0.262:). This PCT discloses the crystalline form II of crystalline Sacubitril calcium salt with characteristic diffraction peaks 1 h corresponding 2::values are 3.2, 7.4, 11.4, 12.9, 16.2, 18.7, 22.5, 23.7 and 27.1(é ě 0.2é 2:).

This PCT discloses the semicrystalline form III of the calcium salt of Sacubitril with characteristic diffraction peaks corresponding 2::values are 4.0 and 4.7 ě 0.2é. This PCT discloses the semicrystalline form IV of the calcium salt of Sacubitril with characteristic diffraction peaks corresponding 2::values are 4.6; 6.4 and 14.3 ě 0.2. This PCT publication also discloses the comparative stability study of crystalline Sacubitril calcium salt and crystal line Sacubitril I sodium salt T his study shows a sample of the calcium salt of Sacubitril I (form I) upon exposing to the temperature of 80éC at 0% relative humidity (R H) and 75% R H for 3 days. The crystalline state of the calcium salt of Sacubitril did not change during this time period. The same experiment was also conducted with the sodium salt of Sacubitril, which exhibited a high rate of polymorph instability and at 75% RH the sample became completely amorphous (see Table 1).

TABLE 1 Stability of solid forms of sacubitril salts Result after loading of the Result after loading of the sample X-ray powder analysis sample at 80° C./0% RH sample at 80° C./75% RH calcium salt of sacubitril Crystalline sample (form I) Crystalline sample(form I)I Crystalline sample(form I)I sodium salt of sacubitril Semicrystalline phase Semicrystalline phase Amorphous sample

Thus there is a need to develop stable crystalline polymorphs of Sacubitril sodium, with the process involving be simple and cost-effective.

PCT Publication No. WO 2007056546 A1 discloses crystalline Valsartan disodium salt, but does not disclose any analytical data or process for preparation of the same;

PCT Publication No. WO 200206253 A1 discloses amorphous and crystalline Valsartan disodium salt; Example 5 of the publication discloses a process for the preparation of the Valsartan disodium salt involving dissolving the valsartan in ethanol, mixing with 2 N sodium hydroxide solution and concentrating by evaporation, and the residue is evaporated a with each of ethanol and ethyl acetate. The white residue is stirred in hot acetonitrile and filtered by suction at room temperature. Drying in a high vacuum at 80éC, overnight yields (S)—N-(1-carboxy-2-methyl-prop-1-yl)-N-pentanoyl-N-[2′-(1 Htetrazol-5-yl)-biphenyl-4-ylmethyl]-amine disodium salt as a white powder. Melting point from 260éC, brownish discolouration at 295éC. There is no reference provided for the nature of the product whether it is amorphous or crystalline.

Example 11 of the same PCT publication No. WO 200206253 discloses a process for the preparation of the Hydrate of valsartan disodium salt (2.4 ě 1.0 mole H2O). A solution was valsartan in isopropanol was added 2N sodium hydroxide solution dropwise at ca. 25éC. The clear solution (pH 7.2) was concentrated under vacuum at ca.40éC. The amorphous residue of the disodium salt was suspended in 100 ml of isopropanol, and water was removed by concentrating under vacuum once more at ca. 40éC and degassing. The amorphous residue was suspended in 75 ml of acetone and 2 ml of water at ca. 40éC. At ca. 25-30éC, 200 ml of tert.-butyl methyl ether were added, whereby constituents that were initially smeary were gradually transformed into a crystalline suspension. After stirring, over night at ca. 25éC, the suspension was cooled to 10éC and after ca. 1 hour was filtered by suction whilst excluding atmospheric moisture. Washed with 20 ml of tert.-butyl methylether. The moist filter cake was dried over night at ca. 30 mbar and at 30éC. A colourless, slightly hygroscopic crystal powder was obtained. There is no X RD diffractogram provided but 2::values with intensity are provided as below.

2θ Intensity 4.7 strong 9.1 strong 13.3 weak 13.7 weak 15.6 medium 16.4 medium 17.2 medium 17.9 medium 18.7 medium 19.6 medium 21.3 medium 21.9 medium 22.8 strong 24.0 weak 24.9 weak 25.5 weak 26.5 medium 26.8 weak 27.3 weak 27.8 weak 28.6 weak 29.4 weak 29.9 medium

The above process as described is laborious and also not cost effective. Also as disclosed in the example 11 the crystalline valsartan disodium hydrate is hygroscopic and hence might not be stable and shelf life would get affected. Thus there is a need to develop stable crystalline polymorphs of valsartan disodium, with the process involving be simple and cost-effective.

A U.S. Pat. No. 8,877,938 B2 discloses Trisodium [3-((1S,3R)-1-biphenyl-4-ylmethyl-3-ethoxycarbonyl-1-butylcarbamoyl) propionate-(S)-3→methyl-2→(pentanoyl{2↓-(tetrazol-5-ylate)biphenyl-4→ylmethyl}amino)butyrate] hemipentahydrate in crystalline form.

EP Patent application no. 2340828 discloses the Trisodium [3-((1S,3R)-1-biphenyl-4-ylmethyl-3-ethoxycarbonyl-1-butylcarbamoyl) propionate-(S)-3→methyl-2→(pentanoyl{2↓-(tetrazol-5-ylate)biphenyl-4→yl methyl}amino)butyrate] in amorphous form.

CN 105461647 application discloses sodium [3-((1S,3R)-1-biphenyl-4-ylmethyl-3-ethoxycarbonyl-1-butylcarbamoyl) propionate-(S)-3→methyl-2→(pentanoyl{2↓-(tetrazol-5-ylate)biphenyl-4-ylmethyl}amino)butyrate] in,

and ∴amorphous form.

PCT Publication No. WO 2007056546 A1 discloses amorphous sacubitril valsartan trisodium and various processes for preparation thereof.

The above process as described is laborious and also not cost effective. Thus there is a need to develop stable amorphous form of sacubitril valsartan trisodium, with the process involving be simple and cost-effective.

SUMMARY OF THE INVENTION

One aspect of the present invention is to provide a crystalline Form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt and a process for the preparation thereof.

Second aspect of the present invention is to provide a process for preparation of crystalline Form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt by dissolving N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt in a suitable solvent, stirring at elevated temperature for a suitable time followed by cooling to an ambient temperature followed by isolation of crystalline Form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt.

Third aspect of the present invention is to provide a crystalline Form B2 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt and a process for the preparation thereof.

Fourth aspect of the present invention relates to a process for preparation of crystalline Form B2 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt by acidifying N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester calcium salt into free acid using aq. HCl in a suitable solvent Reacting the N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester with a suitable base in a suitable solvent, stirring at ambient temperature for a suitable time followed by a cooling to an ambient temperature followed by isolation of crystalline Form B2 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt.

Fifth aspect of the present invention is to provide processes for the preparation of crystalline Form B of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt.

Sixth aspect of the present invention relates to a process for preparation of crystalline Form B of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt by acidifying N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester calcium salt into free acid using aq. HCl in a suitable solvent Reacting the N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester with a suitable base in a suitable solvent, stirring at ambient temperature for a suitable time followed by cooling to an ambient temperature followed by isolation of crystalline Form B of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt.

Seventh aspect of the present invention is to provide a crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt.

Eighth aspect of the present invention is to provide a crystalline Form B of (S)—N-(1-, Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt from amorphous (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt.

Ninth aspect of the present invention is to provide a crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-A mine disodium salt by dissolving amorphous(S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-A mine in a suitable solvent in presence of a suitable sodium source, stirring at an elevated temperature, removal of the solvent azeotropically followed by isolation of crystalline Form B of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt.

Tenth aspect of the present invention is to provide crystalline Form P of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt from amorphous (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine using an aromatic hydrocarbon as the solvent.

Eleventh aspect of the present invention is to provide crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-A mine di sodium salt from amorphous (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine using a sodium source and a suitable solvent.

Twelfth aspect of the present invention relates to process for preparation of amorphous form of sacubitril valsartan trisodium by dissolving sacubitril valsartan trisodium in a suitable solvent.

Thirteenth aspect of the present invention relates to process for preparation of amorphous form of sacubitril valsartan trisodium by acidifying calcium salt of sacubitril to sacubitril free acid and treating with sodium hydroxide and valsartan in presence of a suitable solvent.

Fourteenth aspect of the present invention relates to process for preparation of amorphous solid dispersion of sacubitril valsartan trisodium with an excipient by acidifying calcium salt of sacubitril to sacubitril free acid and treating with sodium hydroxide, excipient and valsartan in presence of a suitable solvent.

The term “suitable solvent” used in the present invention is selected from, but not limited to ‘aliphatic hydrocarbon solvents” such as heptane, hexane, pentane, acetone, methylethyl ketone, methylisobutyl ketone, ethyl acetate, methyl acetate, isopropyl acetate, n-butyl acetate, methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, tert-butanol and the like. “poly hydroxy alkanes” such as ethylene glycol, propane 1,2 diol, propane 1,3 diol, butane 1,2 diol, butane 2,3 diol, aromatic hydrocarbon solvents such as toluene, benzene and the like.

The term ‘sodium source_used in the present invention is selected from, but not limited to sodium hydroxide, sodium carbonate, sodium bicarbonate, sodium methoxide, sodium ethoxide and the like.

The term ‘excipient_used in the present invention is selected from, but not limited to microcrystalline cellulose, hydroxypropyl methyl cellulose, silicon dioxide, hydroxypropyl cellulose, cellulose acetate, mannitol, sorbitol and the like.

BRIEF DESCRIPTION OF THE FIGURES

In order that the disclosure may be readily understood and put into practical effect, reference will now be made to exemplary embodiments as illustrated with reference to the accompanying figures. The figures together with a detailed description below, are incorporated in and form part of the specification, and serve to further illustrate the embodiments and explain various principles and advantages, in accordance with the present disclosure wherein:

FIG. 1 Illustrates the PXRD pattern of crystalline Form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt. The peak list is provided in Table 2.

FIG. 2: Illustrates the PXRD pattern of crystalline Form B2 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt. The peak list is provided in Table 3.

FIG. 3: Illustrates the PXRD pattern of crystalline Form B of N-(3-carboxyl-1-, oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt. The peak list is provided in Table 4.

FIG. 4: Illustrates the PXRD pattern of crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-A mine disodium salt. The peak list is provided in Table 5.

FIG. 5: Illustrates the IR pattern of crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-A mine disodium salt. The peak list is provided in Table 6.

FIG. 6: Illustrates the PXRD of crystalline Form P of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine a disodium salt. The peak list is provided in Table 7.

FIG. 7: Illustrates the PXRD pattern of amorphous form of Sacubitril valsartan trisodium complex.

The method of analysis of the compounds represented in the figures as above are as below:

PXRD Analysis

About 300 mg of powder sample was taken onto the sample holder and was tightly packed on the sample holder uniformly by means of glass slide and Powder X-ray diffraction was recorded on Bruker D8 Advance diffractometer (Bruker-AXS, Karlsruhe, Germany) using Cu—K X-radiation (≅=1.5406

) at 40 kV and 30 mA powder.X-ray diffraction patterns were collected over the 2::range 3-506 at a scan rate of 1é/min.

DSC Analysis

DSC was performed on a Mettler Toledo DSC 822e module. 4-6 mg of sample was placed in crimped but vented aluminium sample pans. The temperature range was from 30-250 éC @ 10 éC/min. Samples were purged by a stream of nitrogen flowing at 80 mL/min.

IR Anlaysis

IR was performed on a Fisher Scientific (NICOLET-iS50-FTIR). About 5 mg of sample was spread over the region of diamond AT R sampling station and collected the sample spectrum between 4000 cm-1 to 400 cm-1 to obtain a spectrum of suitable intensity (above 60% transmission at 2000 cm-1).

DETAILED DESCRIPTION OF THE INVENTION

The term “suitable solvent” used in the present invention is selected from, but not limited to “ester solvents” such as ethyl acetate, methyl acetate, isopropyl acetate, n-butyl acetate and the like; “ether solvents” such as tetrahydrofuran, dimethyl ether, diethyl ether, diisopropyl ether, methyl tert-butyl ether(MTBE), 1,4-dioxane and the like; “hydrocarbon solvents” such as toluene, hexane, heptane, pet ether, xylene, cyclohexane and the like; “polar aprotic solvents” such as dimethyl acetamide, dimethylsulfoxide, dimethylformamide, N-methyl-2-pyrrolidone and the like; “ketone solvents” such as acetone, methylethyl ketone, methylisobutyl ketone and the like; “alcoholic solvents” such as methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, tert-butanol and the like; “chlorinated solvents” such as di chloromethane, chloroform, dichloroethane, carbon tetrachloride and the like; “nitrile solvents” such as acetonitrile, butyronitrile, isobutyronitrile and the like; “polar solvent” such as water or mixtures thereof; ‘aliphatic hydrocarbon solvents” such as heptane, hexane, pentane and the like; “aliphatic hydrocarbon solvents” such as acetone, methylethyl ketone, methylisobutyl ketone, ethyl acetate, methyl acetate, isopropyl acetate, n-butyl acetate, methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, tert-butanol and the like. “poly hydroxy alkanes” such as ethylene glycol, propane 1,2 diol, propane 1,3 diol, butane 1,2 diol, butane 2,3 diol, aromatic hydrocarbon solvents such as toluene, benzene and the like.

The term ‘sodium source_used in the present invention is selected from but not limited to sodium hydroxide, sodium carbonate, sodium bicarbonate, sodium methoxide, sodium ethoxide and the like.

The embodiments of the present invention are further described using specific examples herein after. The examples are provided for better understanding of certain embodiments of the invention and not, in any manner, to limit the scope thereof. Possible modifications and equivalents apparent to those skilled in the art using the teachings of the present description and the general art in the field of the invention shall also form the part of this specification and are intended to be included within the scope of it.

Example 1: Preparation of Crystalline Form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-3 (p-phenylphenylmethyl)-4-amino-2R-methyl Butanoic Acid Ethyl Ester Sodium Salt

A morphous Sacubitril sodium (1 g) in methyl isobutyl ketone (5 mL) was stirred for 10 min at 25-30 éC. The temperature of reaction mass was raised to 40-45 éC to get the clear solution. Stirred the reaction mass for 1 hr at 40-45° C. During stirring, clear solution gradually transformed to crystalline suspension. The suspension was then cooled to 25-30 éC. The suspension was stirred at same temperature for 60 min. The solid was collected by filtration and dried for 12 hrs at 30-35{tilde over (e)}C under vacuum to yield N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt as crystalline Form B1.

The PXRD pattern of Form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt is shown in FIG. 1.

IR (cm⁻¹): 514.62, 603.40, 651.26, 695.70, 733.58, 760.93, 819.75, 850.60, 936.35, 1008.03, 1025.23, 1085.23, 1159.20, 1190.94, 1265.08, 1409.04, 1487.36, 1562.78, 1646.99, 1721.91, 2936.16, 2977.43, 3336.91.

Example 2: Preparation of Crystalline Form B2 of N-(3-Carboxyl-1-Oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl Butanoic Acid Ethyl Ester Sodium Salt

Sacubitril calcium (25 g.) in ethyl acetate was added 5 N HCl to adjust the pH to 1 to 1.5 and stirred for 10 to 20 minutes. The reaction mass was diluted with water and layers were separated. The aqueous layer was extracted with ethyl acetate, organic layers were, combined and concentrated to yield Sacubitril free acid. The concentrated mass of Sacubitril free acid, was added MEK (Methyl ethyl ketone) (3.0 vol.) and 0.5N NaOH (0.9 eq). The reaction mass was stirred for 2 hrs at 25° C. The reaction mass was concentrated and given acetonitrile strip off. MDC (3.0 vol.) and Toluene (6.0 vol.) were added to the residue and partially concentrated at 50° C. to 5.0 vol. stage and then added n-Heptane (5.0 vol.). Stirred the obtained solid for 1 hour. Filtered and washed the solid with n-heptane. Dried the Sacubitril sodium under vacuum at 50° C.

The PXRD pattern of Form B2 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt is shown in FIG. 2.

IR (cm⁻¹): 497.75, 552.98, 608.37, 651.29, 692.67, 732.22, 760.91, 812.97, 839.37, 872.29, 909.96, 933.29, 1015.49, 1099.64, 1128.13, 1162.15, 1189.91, 1242.81, 1270.88, 1296.59, 1372.08, 1416.31, 1434.94, 1487.75, 1523.64, 1564.18, 1647.96, 1708.70, 1731.66, 2916.44, 2978.88, 3030.21, 3351.01.

Example 3: Preparation of Crystalline Form B of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl Butanoic Acid Ethyl Ester Sodium Salt

Sacubitril sodium amorphous (1 g) was added to n-heptane (50 mL) and stirred for 10 min at 25-30 éC. The reaction mass temperature was slowly raised to 90-95 éC and water was removed azeotropically. During azeotropic distillation, clear solution gradually transformed to crystalline suspension. The suspension was cooled to 25-30° C. The suspension was stirred at same temperature for 15-30 min. The solid was collected by filtration and dried for 12 hrs at 30-35éC under vacuum to yield N-(3-carboxyl-1-t, oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt as crystalline Form B.

Example 4: Preparation of crystalline Form B of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl Butanoic Acid Ethyl Ester Sodium Salt

Sacubitril calcium (1.0 Kg.) in ethyl acetate (7.0 Vol) was added 5 N HCl to adjust to pH 1 to 1.5 and stirred for 10 to 20 minutes. The reaction mass was diluted with water and aqueous layers were separated. The aqueous layer was extracted with ethyl acetate, organic layer were combined and concentrated to yield sacubitril free acid. The concentrated mass of sacubitril free acid, was added n-heptane (10.0 V) and aq NaOH (0.9 eq.) and stirred for h, 10 min at 25-30 éC. The reaction mass temperature was slowly raised to 90-95 éC and water was removed azeotropically. The clear solution gradually transformed to crystalline suspension. The suspension was cooled to 25-30 éC. The suspension was stirred at same temperature for 15-30 min. The solid was collected by filtration and dried for 12 hrs at 30-35 éC under vacuum to yield 0.8 Kg of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt as crystalline form B.

The PXRD pattern of crystalline Form B of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt is shown in FIG. 3.

Example 5: Preparation of crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine Disodium Salt

(S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt (3.0 g) was taken in to a RBF and MIBK (Methyl iso butyl ketone) (150 ml) was added. The temperature of reaction mass was slowly raised to reflux and water was removed azeotropically. During azeotropic distillation, the clear solution gradually transformed to crystalline suspension. The suspension thus formed was cooled to 85-90° C. The suspension mass was stirred at same temperature for 15-30 min.

Filtered the reaction mass. The wet cake was dried for 10-12 hr at 30-35° C. under vacuum to yield crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt (2.5 g).

Example 6: Preparation of crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine Disodium Salt

To a solution of amorphous (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-A mine (5.0 g) in 250 ml MIBK (Methyl iso butyl ketone) was added 50% aq. sodium hydroxide (0.96 g.) solution. The temperature of reaction mass was raised to reflux and water was removed azeotropically. During azeotropic distillation, clear solution gradually transformed to crystalline suspension. The suspension was cooled to 85-90° C. The suspension mass was stirred at same temperature for 15-30 min. Filtered the reaction mass. The wet cake was dried for 10-12 hr at 30-35° C. S under vacuum to yield crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-A mine disodium salt (4.48 g).

Example 7: Preparation of crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine Disodium Salt

To a solution of amorphous (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine (1.0 Kg) in MIBK (10 Vol.) (Methyl iso butyl ketone) was added 30% Sodium Methoxide solution in methanol solution (0.88 Kg). The temperature of reaction mass was raised to reflux and methanol was removed azeotropically. The suspension was maintain under reflux for around 2 hr. The suspension was cooled to around 25 to 30° C. The suspension mass was stirred at same temperature for 15-30 min. Filtered the reaction mass. The wet cake was dried for 10-12 hr at 65 to 80° C. under vacuum to yield 0.88 Kg of crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt. The PXRD pattern of Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt is shown in FIG. 4.

IR (cm⁻¹): 561.00, 669.42, 746.37, 760.47, 785.74, 814.68, 861.08, 942.02, 974.87, 1011.05, 1101.83, 1137.16, 1176.23, 1203.48, 1265.48, 1303.84, 1359.14, 1405.62, 1459.74, 1507.88, 1618.07, 2871.76, 2931.82, 2960.36, 3406.61.

The IR pattern of crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-A mine disodium salt is shown in FIG. 5.

Example 8: Preparation of crystalline Form P of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine Disodium Salt

To a solution of amorphous (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-A mine (2.0 g) in 100 ml toluene was added 50% aq. sodium hydroxide (0.38 g.) solution. The temperature of reaction mass was raised to reflux and water was removed azeotropically. During azeotropic distillation, clear solution gradually transformed to crystalline suspension. The suspension was cooled to 85-90° C. The suspension mass was stirred at same temperature for 15-30 min. Filtered the reaction mass. The wet cake was dried for 10-12 hr at 30-35° C. under vacuum to yield crystalline Form P of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine di sodium salt (1.81 g).

The PXRD pattern of Form P of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt is shown in FIG. 6.

IR (cm⁻¹): 561.01, 744.43, 760.41, 941.64, 975.11, 1013.32, 1105.33, 1135.85, 1175.04, 1207.25, 1262.49, 1361.28, 1404.28, 1459.85, 1507.61, 1629.05, 2871.74, 2959.93, 3420.93.

Example 9: Preparation of Amorphous Form of Sacubitril Valsartan Trisodium

Trisodium Sacubitril valsartan (1.0 g.) was dissolved in a mixture of Heptane (50 ml) and Ethylene glycol (0.22 g.) mixture under reflux temperature. Removal of water from the reaction mass was done by azeotropic distillation followed by stripping off five times with fresh Heptane. The suspension was cooled to 25-30° C. The suspension mass was stirred at same temperature for 15-30 min. Filtered the reaction mass. The wet cake was dried for 10-12 hr at 30-35° C. under vacuum to yield Amorphous Form of Sacubitril valsartan trisodium Moisture Content 2.38%.

Example 10: Preparation of Amorphous Form of Sacubitril Valsartan Trisodium

Trisodium Sacubitril valsartan (1.0 g.) was dissolved in a mixture of Heptane (50 ml) and Ethylene glycol (0.11 g.) mixture under reflux temperature. Removal of water from the reaction mass was done by azeotropic distillation followed by stripping off five times with a fresh Heptane. The suspension was cooled to 25-30° C. The suspension mass was stirred at same temperature for 15-30 min. Filtered the reaction mass. The wet cake was dried for 10-12 hr at 30-35° C. under vacuum to yield Amorphous Form of Sacubitril valsartan trisodium Moisture Content 1.35%.

Example 11: Preparation of Amorphous Form of Sacubitril Valsartan Trisodium

Trisodium Sacubitril valsartan (1.0 g.) was dissolved in a mixture of Heptane (50 ml) and Propane 1,2 diol (0.27 g.) mixture under reflux temperature. Removal of water from the reaction mass was done by azeotropic distillation followed by stripping off five times with fresh Heptane. The suspension was cooled to 25-30° C. The suspension mass was stirred at same temperature for 15-30 min. Filtered the reaction mass. The wet cake was dried for 10-12 hr at 30-35° C. under vacuum to yield Amorphous Form of Sacubitril valsartan trisodium Moisture Content 2.48%.

Example 12: Preparation of Amorphous Form of Sacubitril Valsartan Trisodium

Trisodium Sacubitril valsartan (1.0 g.) was dissolved in a mixture of Heptane (50 ml) and t, Propane 1,2 diol (0.13 g.) mixture under reflux temperature. Removal of water from the reaction mass was done by azeotropic distillation followed by stripping off five times with fresh Heptane. The suspension was cooled to 25-30° C. The suspension mass was stirred at same temperature for 15-30 min. Filtered the reaction mass. The wet cake was dried for 10-12 hr at 30-35° C. under vacuum to yield Amorphous Form of Sacubitril valsartan trisodium Moisture Content 2.46%.

Example 13: Preparation of Amorphous Form of Sacubitril Valsartan Trisodium

Trisodium Sacubitril valsartan (1.0 g.) was dissolved in a mixture of Heptane (50 ml) and Butane 2,3 diol (0.32 g.) mixture under reflux temperature. Removal of water from the reaction mass was done by azeotropic distillation followed by stripping off five times with fresh Heptane. The suspension was cooled to 25-30° C. The suspension mass was stirred at same temperature for 15-30 min. Filtered the reaction mass. The wet cake was dried for 10-12 hr at 30-35° C. under vacuum to yield Amorphous Form of Sacubitril valsartan trisodium, Moisture Content 2.2%.

Example 14: Preparation of Amorphous Form of Sacubitril Valsartan Trisodium

Trisodium Sacubitril valsartan (1.0 g.) was dissolved in a mixture of Heptane (50 ml) and Butane 2,3 diol (0.16 g.) mixture under reflux temperature. Removal of water from the reaction mass was done by azeotropic distillation followed by stripping off five times with fresh Heptane. The suspension was cooled to 25-30° C. The suspension mass was stirred at same temperature for 15-30 min. Filtered the reaction mass. The wet cake was dried for 10-12 hr at 30-35° C. under vacuum to yield Amorphous Form of Sacubitril valsartan trisodium. Moisture Content 1.8%.

Example 15: Preparation of Amorphous Form of Sacubitril Valsartan Trisodium

To a solution of Sacubitril calcium (1.0 Kg) in ethyl acetate (7.0 L) added 2N HCl to neutralize at 15-20éC. Organic layer was washed with dil. HCl followed by water and brine solution. The organic layer was added with Valsartan (0.97 Kg), ethyl acetate and aq. Sodium hydroxide solution (0.64 Kg). Distilled off the ethyl acetate and reaction mass was stripped of with ethyl acetate thrice (2 L×3). The reaction mass was added with ethyl acetate (5 L), heptane (25 L) and propanediol (0.053 Kg) to the reaction mass at 25-30éC. The reaction mass was heated to reflux. The solvent in the reaction mass was partially (15.0 Vol.) distilled off and reaction mass was stripped off with heptane thrice. Maintain the reaction mass at reflux temperature for an hour. Cool the reaction mass to 25-30éC. Filtered, off the product and washed the wet cake with heptane. The product was dried at 60éC to yield (1.69 Kg) of Amorphous Form of Sacubitril valsartan trisodium.

The PXRD pattern of amorphous form of Sacubitril valsartan trisodium complex is shown in FIG. 7.

Example 16: Preparation of Amorphous Solid Dispersion of Trisodium Sacubitril Valsartan with Silicified Micro Crystalline Cellulose

To a solution of Sacubitril calcium in ethyl acetate (7.0 vol) added 2N HCl (2.5 vol) to neutralize at 15-20 éC. Organic layer was washed with dil. HCl followed by water and brine a solution. The organic layer was added with Valsartan, ethyl acetate (13 vol) and aq. Sodium hydroxide solution (3.0 eq) followed by silicified micro crystalline cellulose (100% w/w) to the reaction mass. Distil off the ethyl acetate and strip of with ethyl acetate trice in order to control the moisture. Add ethyl acetate (5 vol), heptane (25 vol) and propanediol (0.3 eq) to the reaction mass at 25-30 éC. H eat the reaction mass to the reflux. Remove the solvent (15 vol) and add same amount of heptane trice in order to get rid of ethyl acetate and moisture. Maintain the reaction mass at reflux temperature for an hour. Cool the reaction mass to 25-30éC. Filter the product under nitrogen atmosphere and wash the wet cake with heptane. Dry the product at 60éC.

Example 17: Preparation of Amorphous Solid Dispersion of Trisodium Sacubitril Valsartan with Mannitol

To a solution of Sacubitril calcium in ethyl acetate (7 vol) added 2N HCl (2.5 vol) to neutralize at 15-20 éC. Organic layer was washed with dil. HCl followed by water and brine solution. The organic layer was added with Valsartan, ethyl acetate (13 vol) and aq. Sodium hydroxide solution (3.0 eq) followed by mannitol (100% w/w) to the reaction mass. Distil off the ethyl acetate and strip of with ethyl acetate trice in order to control the moisture. Add ethyl acetate (5 vol), heptane (25 vol) and propanediol (0.3 eq) to the reaction mass at 25-30éC. Heat the reaction mass to the reflux. Remove the solvent (15 vol) and add same amount of heptane trice in order to get rid of ethyl acetate and moisture. Maintain the reaction mass at reflux temperature for an hour. Cool the reaction mass to 25-30éC. Filter, the product under nitrogen atmosphere and wash the wet cake with heptane. Dry the product at 60éC.

TABLE 2 Num. Gonio d Int I/Imax 1 3.7243 23.7054 555 100.0 2 7.4329 11.8836 89 16.0 3 9.1473 9.66013 97 17.5 4 10.4323 8.4729 85 17.1 5 10.8514 8.1466 89 16.0 6 11.8532 7.46025 116 21.0 7 12.5970 7.02137 122 22.0 8 15.0292 5.89011 91 16.5 9 16.4338 5.38968 93 16.8 10 16.5870 5.34024 97 17.5 11 17.2831 5.12671 183 33.0 12 18.3937 4.8196 215 38.7 13 19.2247 4.61308 173 31.2 14 19.9610 4.44456 249 45.0 15 20.7254 4.28233 203 36.7 16 22.5652 3.93717 125 22.5 17 23.5987 3.76703 103 18.6 18 27.6767 3.22054 79 14.2

TABLE 3 Num. Gonio d Int I/Imax 1 3.2312 27.322 421 100.0 2 3.5878 24.6068 257 61.0 3 3.9779 22.1946 143 34.0 4 6.3799 13.6427 114 27.2 5 7.3164 12.0729 106 25.1 6 7.9535 11.1072 51 12.2 7 9.0387 9.77589 57 13.6 8 10.7365 8.23354 79 18.7 9 12.0804 7.32041 112 26.6 10 12.8855 6.96479 89 21.2 11 13.8766 6.37662 64 15.3 12 15.7226 5.63187 61 14.6 13 16.6555 5.31844 94 22.4 14 17.7643 4.98892 131 31.0 15 18.5071 4.79032 191 35.8 16 20.0250 4.43049 285 67.7 17 20.9639 4.23414 175 41.6 18 21.7151 4.08934 128 30.5 19 22.4538 3.95644 103 24.5 20 23.9198 3.71719 93 22.2 21 28.6590 3.11235 75 17.9

TABLE 4 Num. Gonio d Int I/Imax 1 3.1506 28.0207 486 100.0 2 5.3444 16.5222 87 18.0 3 6.2688 14.0878 193 39.7 4 7.2662 12.1561 105 21.7 5 7.8370 11.272 62 12.7 6 8.8629 9.93944 50 10.3 7 9.3202 9.48125 48 9.9 8 10.6503 8.29994 211 43.4 9 11.4873 7.69703 111 22.9 10 11.9856 7.37808 142 29.2 11 12.4824 7.08553 106 21.9 12 12.7793 6.92159 130 26.7 13 13.8192 6.40298 74 15.3 14 14.8691 5.95316 65 13.5 15 15.6224 5.66776 76 15.6 16 16.5290 5.35887 125 25.8 17 17.6209 5.02919 101 20.8 18 18.3486 4.83133 110 22.7 19 19.9596 4.44486 387 79.8 20 20.8753 4.25192 183 37.6 21 21.5908 4.1126 175 36.0 22 22.2297 3.99583 127 26.1 23 23.8380 3.72975 111 22.8 24 24.9636 3.56407 76 15.7 25 26.1809 3.40105 96 19.8 26 26.9827 3.30178 104 21.4 27 28.5473 3.12427 73 15.0

TABLE 5 Num. Gonio d Int I/Imax 1 6.4897 13.6089 1578 100.0 2 8.4028 10.5142 553 25.1 3 8.6583 10.2045 261 16.5 4 9.6388 9.16859 408 25.9 5 10.9027 8.10837 136 8.6 6 12.0513 7.33802 86 5.5 7 12.9832 6.81334 291 18.5 8 13.9832 6.36451 117 7.4 9 14.3101 6.18442 170 10.8 10 15.0999 5.86269 150 9.5 11 16.0389 5.52149 198 12.5 12 16.4276 5.3917 129 8.2 13 16.8524 5.25676 72 4.6 14 17.4291 5.0841 561 35.6 15 17.9309 4.94292 432 8.3 16 18.7869 4.7185 118 7.5 17 19.6048 4.52449 443 28.1 18 20.3749 4.3552 78 5.0 19 21.1596 4.19543 223 14.1 20 32.444 3.95809 129 8.2 21 23.4998 3.78265 87 5.5 22 23.9252 3.71635 207 13.1 23 24.6215 3.61281 152 9.7 24 25.2398 3.5258 95 6.0 25 25.6671 3.46796 77 4.9 26 26.1507 3.40491 258 16.3 27 27.1518 3.28159 83 5.3 28 27.5500 3.23506 192 12.2 29 27.9903 3.18517 122 7.7 30 28.2779 3.15342 101 6.4 31 30.5421 2.92461 51 3.2 32 31.3701 2.84928 59 3.7 33 32.1253 2.784 56 3.5 34 32.7692 2.73075 58 3.7 35 34.0631 2.62993 117 7.4 36 38.3156 2.34725 42 2.7 37 43.1023 2.09702 40 2.5 38 43.8513 2.06292 43 2.7 39 44.4142 2.03807 44 2.8 40 45.1146 2.00804 42 2.6

TABLE 6 Region: 4000.00 400.00 Absolute threshold:  93.064 Sensitivity:  50 Peak: Position: 561.03 Intensisty: 50.049 Position: 668.93 Intensisty: 56.730 Position: 745.64 Intensisty: 45.266 Position: 760.53 Intensisty: 42.729 Position: 765.81 Intensisty: 67.050 Position: 814.62 Intensisty: 72.450 Position: 860.96 Intensisty: 80.888 Position: 942.05 Intensisty: 86.220 Position: 974.66 Intensisty: 85.575 Position; 1012.21 Intensisty: 76.252 Position: 1103.23 Intensisty: 76.048 Position: 1136.97 Intensisty: 79.897 Position: 1175.76 Intensisty: 75.857 Position: 1204.88 Intensisty: 77.131 Position: 1264.12 Intensisty: 73.793 Position: 1303.37 Intensisty: 76.782 Position: 1358.67 Intensisty: 50.561 Position: 1405.23 Intensisty: 27.136 Position: 1459.75 Intensisty: 29.723 Position: 1508.14 Intensisty: 68.569 Posiition: 1604.03 Intensisty: 13.762 Position: 2671.81 Intensisty: 58.109 Position: 2931.84 Intensisty: 46.836 Position: 2960.31 Intensisty: 34.049 Position: 3415.36 Intensisty: 20.280

TABLE 4 Num. Gonio d Int I/Imax 1 5.2534 16.8083 246 45.5 2 5.6390 15.6598 540 100.00 3 6.6507 13.2797 169 31.3 4 7.3378 12.0377 156 28.9 5 8.1022 10.9036 188 34.8 6 10.3687 8.52477 130 24.0 7 11.1951 7.89722 131 24.3 8 14.4589 6.12112 112 20.8 9 19.6362 4.51735 128 23.7 10 23.1438 3.84003 103 19.0 11 25.7551 3.4563 88 16.3 

The invention claimed is:
 1. Crystalline form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester (sacubitril) sodium salt characterized by an X ray powder diffraction (X RPD) spectrum having peak reflections at about 3.7, 7.4, 11.8, 12.6, 16.4, 16.6, 17.3, 18.4, 19.2, 19.9, 20.7, 22.5 and 23.5±0.2 degrees 2 theta, as shown in FIG.
 1. 2. Crystalline form B2 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester (sacubitril) sodium salt characterized by an X-ray powder diffraction (X RPD) spectrum having peak reflections at about 3.2, 3.6, 4.0, 6.4, 12.1, 17.8, 18.5, 20.0, 21.0 and 21.7±0.2 degrees 2 theta, as shown in FIG.
 2. 3. A process for the preparation of crystalline form B1 of claim 1, N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester (sacubitril) sodium salt, the process comprising: a) heating a solution of sacubitril sodium salt in methyl isobutyl ketone at elevated temperature; b) cooling the solution from step a); and c) isolating the crystalline form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl (sacubitril) sodium salt.
 4. A process for the preparation of crystalline form B2 of claim 2, N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester (sacubitril) sodium salt, the process comprising: a) converting sacubitril calcium salt into sacubitril free acid in presence of HCl and in ethyl acetate solvent at elevated temperature; b) treating sacubitril from step a) with sodium hydroxide and c) isolating the crystalline form B2 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester (sacubitril) sodium salt. 